Background: The outbreak of coronavirus disease 2019 (COVID-19) and SARS-CoV-2 have placed unprecedented challenges on hospital environmental hygiene and medical staffs protection. It is crucial to assess hospital environmental hygiene to understand the most important environmental issues for controlling the spread of 2019-nCoV in hospitals. Objective: To detect the presence of the COVID-19 in the air and on the surfaces of the guide station, fever clinic, and isolation areas, and the close contacts medical staffs in the First Hospital of Jilin University. Methods: Viruses in the air were collected by natural sedimentation and air particle sampler methods. Predetermined environmental surfaces were sampled using swabs at seven o'clock in the morning before disinfection. The samples from close contacts medical staffs were throat swab samples. Quantitative real-time PCR methods were used to confirm the existence of COVID-19 pathogens. Results: Viruses could be detected on the surfaces of the nurse station in the isolation area with suspected patients and in the air of the isolation ward with an intensive care patient. Conclusion: Comprehensive monitoring of hospital environmental hygiene during pandemic outbreaks is conducive to the refinement of hospital infection control. It is of great significance to ensure the safety of medical treatment and the quality of hospital infection control through the monitoring of environmental hygiene.