The recently launched high-throughput assays for the detection of antibodies against SARS-CoV-2 may change the diagnostic strategies for COVID-19. This study aimed at investigating the performance of three novel high-throughput assays on Abbott Architect, Roche Cobas, and DiaSorin Liaison platforms. In addition, we evaluated a rapid lateral flow test from Dynamiker Biotechnology. A panel consisting of 133 samples including 100 pre-pandemic samples, 20 samples from SARS-CoV-2 PCR positive individuals and 13 possible crossreactive samples were used. Sensitivity and specificity in this study were equivalent with data from Abbott and Roche but differed from those reported from DiaSorin and Dynamiker Biotechnology. The results suggest that the assays from Abbott and Roche could be considered in clinical use for individual patients.